cAMP Assay:Article Title: Nonpeptide somatostatin type 5 receptor agonists and uses thereof
Article Snippet: .. One example of an intracellular cAMP assay is described below. cAMP Assay Protocol for SST2R: Four days prior to the assay, 5,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 2 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 μg/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.2 mg/mL hygromycin B (GoldBio #31282-04-9). .. cAMP Assay Protocol for SST2R: Four days prior to the assay, 5,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 2 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 μg/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.2 mg/mL hygromycin B (GoldBio #31282-04-9).
Article Title: Nonpeptide somatostatin type 5 receptor agonists and uses thereof
Article Snippet: The time-resolved fluorescent signal is read with a Tecan M1000Pro multiplate reader. .. All data manipulations are in GraphPad Prism v6 or v7. cAMP Assay Protocol for SST5R: Four days prior to the assay, 2,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 5 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 g/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.25 mg/mL G418 Sulfate (GoldBio #108321-42-2). .. All data manipulations are in GraphPad Prism v6 or v7.
Stable Transfection:Article Title: Nonpeptide somatostatin type 5 receptor agonists and uses thereof
Article Snippet: .. One example of an intracellular cAMP assay is described below. cAMP Assay Protocol for SST2R: Four days prior to the assay, 5,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 2 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 μg/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.2 mg/mL hygromycin B (GoldBio #31282-04-9). .. cAMP Assay Protocol for SST2R: Four days prior to the assay, 5,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 2 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 μg/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.2 mg/mL hygromycin B (GoldBio #31282-04-9).
Article Title: Nonpeptide somatostatin type 5 receptor agonists and uses thereof
Article Snippet: The time-resolved fluorescent signal is read with a Tecan M1000Pro multiplate reader. .. All data manipulations are in GraphPad Prism v6 or v7. cAMP Assay Protocol for SST5R: Four days prior to the assay, 2,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 5 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 g/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.25 mg/mL G418 Sulfate (GoldBio #108321-42-2). .. All data manipulations are in GraphPad Prism v6 or v7.
Expressing:Article Title: Nonpeptide somatostatin type 5 receptor agonists and uses thereof
Article Snippet: .. One example of an intracellular cAMP assay is described below. cAMP Assay Protocol for SST2R: Four days prior to the assay, 5,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 2 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 μg/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.2 mg/mL hygromycin B (GoldBio #31282-04-9). .. cAMP Assay Protocol for SST2R: Four days prior to the assay, 5,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 2 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 μg/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.2 mg/mL hygromycin B (GoldBio #31282-04-9).
Article Title: Nonpeptide somatostatin type 5 receptor agonists and uses thereof
Article Snippet: The time-resolved fluorescent signal is read with a Tecan M1000Pro multiplate reader. .. All data manipulations are in GraphPad Prism v6 or v7. cAMP Assay Protocol for SST5R: Four days prior to the assay, 2,000 Chinese hamster ovary cells (CHO-K1, ATCC #CCL-61) stably expressing the human somatostatin receptor subtype 5 are plated in each well of a 96-well tissue culture-treated plate in Ham's F12 growth media (ThermoFisher #10-080-CM) supplemented with 10% donor bovine serum (Gemini Bio-Products #100-506), 100 U/mL penicillin; 100 g/mL streptomycin; 2 mM L-glutamine (Gemini Bio-Products #400-110) and 0.25 mg/mL G418 Sulfate (GoldBio #108321-42-2). .. All data manipulations are in GraphPad Prism v6 or v7.
other:Article Title: April and BAFF inhibitory immunomodulatory proteins and methods of use thereof
Article Snippet: Examples of suitable mammalian host cells include African green monkey kidney cells (Vero; ATCC CRL 1587), human embryonic kidney cells (293-HEK; ATCC CRL 1573), baby hamster kidney cells (BHK-21, BHK-570; ATCC CRL 8544, ATCC CRL 10314), canine kidney cells (MDCK; ATCC CCL 34), Chinese hamster ovary cells (CHO-K1; ATCC CCL61; CHO DG44 (Chasin et al, Som.
Cell Culture:Article Title: A neurotensin receptor type 1-derived pepducin acts as a biased allosteric modulator to regulate target receptor function
Article Snippet: Finally, a 3xHA-hNTSR1 pcDNA3.1 construct was purchased from the Bloomsburg University cDNA Resource Center (Bloomsburg, PA, USA) and provided to GenScript (Piscataway, NJ, USA) as the template for the construction of the 27 NTSR1 ICL mutant cDNAs (ICL Mutants 1–27). .. Chinese hamster ovary cells (CHO-K1, CCL-61 from ATCC, Manassas, VA, USA) were cultured in DMEM-F12 medium supplemented with 20 mmol/L HEPES, 10% FBS, and penicillin (100 U/mL)-streptomycin (100 mg/mL). .. CHO-K1 cells stably expressing hNTSR1 (CHO-hNTSR1, ES-690-C), purchased from PerkinElmer (Montreal, QC, Canada), were cultured in the same conditions but further supplemented with 0.4 mg/mL of geneticin (G418).
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